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B27细胞培养添加剂invitrogen/Gibco(货号17504-044)说明书

  • 产品型号:
  • 产品时间:2017-12-07
  • 简要描述:B27细胞培养添加剂invitrogen/Gibco(货号17504-044)说明书;由上海慧颖生物提供,库存现货,价格优惠,另有N2添加剂促销热卖中,B-27® 添加剂是无血清添加剂,用于海马神经元和其他中枢神经系统(CNS)神经元的生长和保持其短期或长期活性。咨询!
  • 产品简介

B27细胞培养添加剂invitrogen/Gibco(货号17504-044)说明书

 

描述

B-27® 添加剂是无血清添加剂,用于海马神经元和其他中枢神经系统(CNS)神经元的生长和保持其短期或长期活性。B-27® 添加剂是50X 的液体,可以与Neurobasal® 培养基或Neurobasal®-A 培养基组合使用,用于神经细胞培养而不需要再添加饲养层细胞。

B-27® 添加剂适和应用于大多数神经细胞培养。对于特别的应用,我们提供 其他配方的B-27® 添加剂.

质量控制检测 
B-27® 添加剂 进行了18天妊娠的原代大鼠(Sprague Dawley) 的胚胎海马神经元生长检测。 通过了细菌和真菌的检测. B-27® 也做了1X 浓度的内毒素水平测试。 

仅供研究使用。除非另有说明,不得用于任何动物或人类治疗或诊断。

详细说明

B27细胞培养添加剂invitrogen/Gibco(货号17504-044)说明书 常用规格

Cell Type:

Neuronal Cells 

Product Size:

10 ml 

Classification:

Serum-Free 

Other Additives:

Vitamin A, 

Antioxidant Cocktail, 

Insulin 

Purity or Quality Grade:

Cell Culture Grade 

Green Features:

Sustainable packaging 

Shipping Condition:

Dry Ice 

Regulatory Statement:

For Research Use Only. Not for use in diagnostic procedures. 

引文和参考文献 (17)

  • D2 receptors inhibit the secretory process downstream from calcium influx in dopaminergic neurons: implication of K+ channels.

作者

Congar P; Bergevin A; Trudeau LE

杂志

J Neurophysiol (2002) 87:1046-1102

产品用途

Using a culture system in which isolated DAergic neurons establish self-innervating synapses ("autapses") that release both DA and glutamate, we studied the mechanism by which presynaptic D2 receptors inhibit glutamate-mediated excitatory postsynaptic currents (EPSCs). 

ID:

PN64425

  • Decreased synaptic vesicle recycling efficiency and cognitive deficits in amphiphysin 1 knockout mice.

作者

Di Paolo Gilbert; Sankaranarayanan Sethuraman; Wenk Markus R; Daniell Laurie; Perucco Ezio; Caldarone Barbara J; Flavell Richard; Picciotto Marina R; Ryan Timothy A; Cremona Ottavio; De Camilli Pietro;

杂志

Neuron (2002) 33:789-804

产品用途

Primary cultures of cortical neurons were prepared. Cells were maintained up to 3 weeks in Neurobasal/B27 medium at 37°C in a 5% CO2 humidified incubator. 

ID:

PN47418

  • Blocking HES1 expression initiates GABAergic differentiation and induces the expression of p21(CIP1/WAF1) in human neural stem cells.

作者

Kabos Peter; Kabosova Andrea; Neuman Toomas;

杂志

J Biol Chem (2002) 277:8763-8769

产品用途

Resected human fetal brain tissue was placed into ice cold DMEM/F-12 with penicillin/streptomycin for further dissection. The tissue was cut into small pieces and trypsinized (0.02 mg/ml trypsin in versene (Invitrogen) at 37 °C for 10 min. Cell suspension was centrifuged at 400 rpm for 5 min, the pellet was washed once with DMEM/F-12 and plated at a density of 5000-10,000 viable cells/ml in the growth medium DMEM/F-12, B27 supplement (Invitrogen). Cells were washed with PBS (Invitrogen)  

ID:

PN63471

  • Long-term culture of mouse cortical neurons as a model for neuronal development, aging, and death.

作者

Lesuisse Christian; Martin Lee J;

杂志

J Neurobiol (2002) 51:9-23

产品用途

A long-term cell culture system was used to study maturation, aging, and death of cortical neurons. 

ID:

PN60461

  • An improved method for the expression of TRH in serum-supplemented primary cultures of fetal hypothalamic cells.

作者

Joseph-Bravo Patricia; Pérez-Martínez Leonor; Lezama Leticia; Morales-Chapa Claudia; Charli Jean Louis;

杂志

Brain Res Brain Res Protoc (2002) 9:93-104

产品用途

Primary cultures of dispersed cells from fetal nervous tissue are extensively used for studying multiple neuronal properties 

ID:

PN62028

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按细胞系排列的引文

细胞系名称有机体类型组织
SH-Sy5YHumanBrain
Fetal neuronsRatBrain
Neurons (post-natal/adult)RatBrain
Neurons (superior cervical ganglia - SCG)RatBrain
Neurons (cortical)MouseOther
Neural Stem CellsRatBrain

 

 

 

慧颖生物主营:生化试剂、抗体、重组蛋白、细胞生物、分子生物、蛋白研究、微生物、Elisa试剂盒、耗材、标准品、 标准溶液……

abcam、ABI、Axygen、corning、CST、Gibco、Hyclone、invitrogen、merck、Millipore、Qiagen、R&D、Roche、santa、sigma、Thermo等进口品牌……

以下为相关本公司常备现货产品

 

*部分 invitrogen 畅销原装产品

 

 

品牌货号名称规格
invitrogen13778-075Lipofectamine RNAiMAX0.75ml
invitrogen13778-150Lipofectamine RNAiMAX1.5ml
invitrogen11668-027Lipofectamine 2000 Reagent0.75ml
invitrogen11668-019Lipofectamine 2000 Reagent1.5ml
invitrogen15596-026TRlzol ®Reagent100ml
invitrogen15596-018TRlzol ®Reagent200ml
invitrogen10296-010Trizol ®  LS Reagent100ml
invitrogen10296-028Trizol ®  LS Reagent200ml
invitrogen17504-044B-27(黄颜色)10ml
invitrogen12587-010B-27(黄颜色)不含维生素A10ml
invitrogen17502-048N-2  (粉红颜色)5ml
invitrogen18064-014SuperScript® II Reverse Transcriptase10,000U
invitrogen18080-044SuperScript® III Reverse Transcriptase10,000U

 

 

第二部分 HyClone胎牛血清

 

 

品牌货号名称规格
HyCloneSH30370.03Australian Standard Fetal Bovine Serum500ml
HyCloneSH30406.02FOETAL BOVINE SERUM500ml
HyCloneSH30401.01New Zealand Calf Serum500ml
HyCloneSH30066.02FetalClone® II100ml
HyCloneSH30088.03STANDARD FETAL BOVINE SERUM500ml
HyCloneSH30118.02Newborn Bovine Calf Serum, U.S. Origin100ml
HyCloneSH30070.02FETAL BOVINE SERUM100ml
HyCloneSH30087.02COSMIC CALF® SERUM100ml
HyCloneSH30070.03EFETALBOVINESERUM CHARACTERIZSED500ml
100ml
HyCloneSH30070.03FETAL BOVINE SERUM500ml
HyCloneSH30080.03FetalClone® I500ml
HyCloneSH30084.03FOETAL BOVINE SERUM: CERTIFIED AUSTRALIAN500ml
HyCloneSH30396.03FETAL BOVINE SERUM CHARACTERIZED500ml
HyCloneSV30087.02南美源胎牛血清500ml
HyClonesv30087.01南美源胎牛血清100ml
HyCloneSH30071.03FETAL BOVINE SERUM500ml
HyCloneSH30071.02FETAL BOVINE SERUM100ml
HyCloneSH30080.02FetalClone® I100ml
HyCloneSH30070.02EFETAL BOVINE SERUM CHARACTERIZSED100ml
HyCloneSH30079.02FETAL BOVINE SERUM100ml
HyCloneSH30068.02CHARCOAL/DEXTRAN TREATED FBS,
活性炭灭活血清
100ml
HyCloneSH30068.03CHARCOAL/DEXTRAN TREATED FBS,
活性炭灭活血清
500ml
HyCloneSH30066.03FetalClone® II500ml
HyCloneSH30109.03HyClone FetalClone® III500ml
HyCloneSH30088.03STANDARD FETAL BOVINE SERUM500ml
HyCloneSH30088.02STANDARD FETAL BOVINE SERUM100ml
HyCloneSH30079.03FETAL BOVINE SERUM500ml

 

 

第二部分  Gibco胎牛血清

 

 

品牌货号名称规格
Gibco12662-029MSC QUALIFIED FBS 500ML500ML
Gibco10438-026FBS, HI 500ML500ML 
Gibco10439-024FBS, ES CELL QUAL. USDA APPD. 500ML500ML
Gibco10099-141FBS, AUSTRALIAN ORIGIN 500ML500ML 
Gibco26400-044FBS, DIALYZED, USA ORIGIN 500ML500ML
Gibco26140-079FBS QUALIFIED, USA ORIGIN 500ML500ML
Gibco16141-079FBS, ES CELL QUAL, USA ORIGIN 500ML500ML
Gibco16140-071FBS, HI 500ML500ML
Gibco16000-044FBS, CERT, USA ORIGIN 500ML500ML
GibcoC2027050  FBS, URUGUAYAN ORIGIN 500ML
Gibco12657-029巴西胎牛500ml

 

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