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产品名称:Invitrogen 18012021 LARGE FRAGMENT
货 号:18012021
规 格:100 UN
分 类:DNA/RNA 聚合酶
品 牌:Invitrogen
国产/进口:进 口
储存温度:-20°C
特惠价格:致 电
库存状态:现 货
销售商:上海慧颖生物科技有限公司
慧颖生物专业供应Invitrogen 18012021 LARGE FRAGMENT,常备现货,*。主营Life Invitrogen产品,囊括TRlzol ?Reagent、TRIZOL LS Reagent 、B-27添加剂、凝胶填料、常规化学试剂、培养基及原料试剂、各类胎牛血清、马血清等,如有需要,详询。
描述:
DNA Polymerase I, Large (Klenow) Fragment is a DNA polymerase enzyme that lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I, but does exhibit the 5' to 3' DNA polymerase and 3' to 5' exonuclease activities.
Applications:
Fill-in of 5′ overhangs (1). Synthesis of probes by random primers labeling method (2). Sequencing single- and double-stranded DNA (3). Site-directed mutagenesis.
Source:
Purified from E. coli expressing the Klenow fragment on a plasmid.
Performance and Quality Testing:
SDS-PAGE purity; single-stranded and double-stranded endodeoxyribonuclease and self-priming assays; performance evaluated in fill-in reaction.
Unit Definition:
One unit incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template?primer.
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