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Invitrogen 18064-014 SuperScript? II Reverse Transcriptase 同类产品:
品牌 | 名称 | 货号 | 规格 | 市场价格(元) |
Invitrogen | SuperScript? II Reverse Transcriptase | 18064-014 | 10,000 units | 3918 |
Invitrogen | SuperScript? II Reverse Transcriptase | 18064-022 | 2,000 units | 1142 |
Invitrogen | SuperScript? II Reverse Transcriptase | 18064-071 | 4 x 10,000 units | 13860 |
Invitrogen 18064-014 SuperScript? II Reverse Transcriptase 简介:
产品名称:SuperScript? II Reverse Transcriptase
分类:反转录酶
中文名称:反转录酶II
品牌:Invitrogen
货号:18064-014
规格:10,000 units
市场价格:3918元
特惠价格:致电
库存状态:现货
Invitrogen 18064-014 SuperScript? II Reverse Transcriptase 英文描述:
SuperScript? II Reverse Transcriptase (RT) is an improved version of SuperScript? RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations in the RNase H active center to reduce RNase H activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript? II RT superior performance characteristics.
? Yield – Reduced RNase H activity results in greater first-strand cDNA yields
? Size of cDNA – Generation of RT-PCR products up to 12 kb
? Thermostability – Full activity at 42°C
? Flexibility – Uses either total or poly(A)+ RNA
Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product.
Unit Definition: One unit of SuperScript? II is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)?oligo(dT)12-18 as template?primer (3).
Unit Reaction Conditions: 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18, 0.1 mg/ml BSA, and enzyme in 50 μl for 10 min. at 37°C.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Invitrogen 18064-014 SuperScript? II Reverse Transcriptase 相关主要产品:
货号 | 名称 | 规格 |
18064-014 | SuperScript? II Reverse Transcriptase | 10,000U |
18080-044 | SuperScript? III Reverse Transcriptase | 10,000U |
18418-012 | Oligo(dT)12-18 Primer | 25ug |
18418-020 | Oligo(dT)20 Primer | 50ul(50uM) |
10777-019 | RNaseOUTTM Ribonuclease Inhibitor | 5,000U |
18080-051 | SuperScript? III First-Strand | 50 rxn |
Synthesis System for RT-PCR | ||
11668-027 | Lipofectamine TM 2000 Reagent | 0.75ml |
11668-019 | 1.5ml | |
13778-150 | Lipofectamine RNAiMax | 1.5ml |
13778-075 | Lipofectamine RNAiMax | 0.75ml |
15596-026 | TRlzol ?Reagent | 100ml |
15596-018 | TRlzol ?Reagent | 200ml |
10296028 | TRIZOL LS Reagent 200 ml(长方形) | 200ml |
10296010 | TRIZOL LS Reagent 100ml(长方形) | 100ml |
11789020 | BP CLONASE II ENZYME MIX | 20 REACTIONS |
11791020 | LR CLONASE II ENZYME MIX | 20 REACTIONS |
12328040 | AMV | 100 REACTIONS |
(CLON AMV FIRST STR SYNTH KIT) | ||
28025-013 | M-MLV Reverse Transcriptase | 40,000U |
17504-044 | B-27(黄颜色) | 10ml |
12587-010 | B-27(黄颜色)不含维生素A | 10ml |
15090-046 | 2.5% Trypsin (10X), no Phenol Red | 100ml |
15050-065 | 0.25% Trypsin(1X),Phenol Red | 100ml |
15050-057 | 500ml |
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