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Invitrogen 18064-014 SuperScript® II Reverse

  • 产品型号:
  • 产品时间:2024-08-12
  • 简要描述:慧颖生物专业供应Invitrogen 18064-014 SuperScript® II Reverse Transcriptase,10,000U,常备现货,*,咨询!
  • 产品简介

Invitrogen 18064-014 SuperScript? II Reverse Transcriptase 同类产品:

品牌

名称

货号

规格

市场价格(元)

Invitrogen

SuperScript? II Reverse Transcriptase

18064-014

10,000 units

3918

Invitrogen

SuperScript? II Reverse Transcriptase

18064-022

2,000 units

1142

Invitrogen

SuperScript? II Reverse Transcriptase

18064-071

4 x 10,000 units

13860


Invitrogen 18064-014 SuperScript? II Reverse Transcriptase 简介:

产品名称:SuperScript? II Reverse Transcriptase

分类:反转录酶

中文名称:反转录酶II

品牌:Invitrogen

货号:18064-014

规格:10,000 units

市场价格:3918元

特惠价格:致电

库存状态:现货


Invitrogen 18064-014 SuperScript? II Reverse Transcriptase 英文描述:

SuperScript? II Reverse Transcriptase (RT) is an improved version of SuperScript? RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations in the RNase H active center to reduce RNase H activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript? II RT superior performance characteristics.
Yield – Reduced RNase H activity results in greater first-strand cDNA yields
Size of cDNA – Generation of RT-PCR products up to 12 kb
Thermostability – Full activity at 42°C
Flexibility – Uses either total or poly(A)+ RNA
Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product.
Unit Definition: One unit of SuperScript? II is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)?oligo(dT)12-18 as template?primer (3).
Unit Reaction Conditions: 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18, 0.1 mg/ml BSA, and enzyme in 50 μl for 10 min. at 37°C.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.


Invitrogen 18064-014 SuperScript? II Reverse Transcriptase 相关主要产品:

货号

名称

规格

18064-014

SuperScript? II Reverse Transcriptase

10,000U

18080-044

SuperScript? III Reverse Transcriptase

10,000U

18418-012

Oligo(dT)12-18 Primer

25ug

18418-020

Oligo(dT)20 Primer

50ul(50uM)

10777-019

RNaseOUTTM Ribonuclease Inhibitor

5,000U

18080-051

SuperScript? III First-Strand

50 rxn

Synthesis System for RT-PCR

11668-027

Lipofectamine TM 2000 Reagent

0.75ml

11668-019

1.5ml

13778-150

Lipofectamine RNAiMax

1.5ml

13778-075

Lipofectamine RNAiMax

0.75ml

15596-026

TRlzol ?Reagent

100ml

15596-018

TRlzol ?Reagent

200ml

10296028

TRIZOL LS Reagent 200 ml(长方形)

200ml

10296010

 TRIZOL LS Reagent 100ml(长方形)

100ml

11789020

BP CLONASE II ENZYME MIX

20 REACTIONS

11791020

LR CLONASE II ENZYME MIX

20 REACTIONS

12328040

AMV

100 REACTIONS

(CLON AMV FIRST STR SYNTH KIT)

28025-013

M-MLV Reverse Transcriptase

40,000U

17504-044

B-27(黄颜色)

10ml

12587-010

B-27(黄颜色)不含维生素A

10ml

15090-046

2.5% Trypsin (10X), no Phenol Red

100ml

15050-065

0.25% Trypsin(1X),Phenol Red

100ml

15050-057

500ml

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