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人小细胞肺癌细胞;NCI-H69细胞

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  • 产品时间:2017-11-07
  • 简要描述:上海慧颖生物专业供应人小细胞肺癌细胞;NCI-H69细胞, 慧颖生物拥有完善的细胞库和管理体系,细胞技术20%为博士学历、40%为硕士学历,自公司成立以来,慧颖细胞库携手合作单位,以每月平均近100株的量向高校、医院、研究所等提供优质细胞株细胞系。
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人小细胞肺癌细胞;NCI-H69细胞

数量:大量
生长状态:悬浮生长,多细胞聚集
细胞形态:其他
年限:55 years adult
ATCC Number:HTB-119™
相关疾病:小细胞肺癌
是否是肿瘤细胞:1
物种来源:
运输方式:冻存运输
器官来源:
规格:0.1ml

收到人小细胞肺癌细胞;NCI-H69细胞后的处理:

1)收到细胞后,首先观察瓶身是否完好(注意有无缝隙,裂痕)。

2)显微镜下观察细胞的生长状况,有无细胞漂浮。

3)用新洁尔灭消毒瓶口及瓶身。

4)打开瓶口,再次用新洁尔灭消毒瓶口(可能会有液体溢出,注意不要碰到液体),酒精灯烤瓶口消毒。

5)将培养液转移至50ml离心管或广口瓶中(若细胞漂浮严重,离心培养基,1000g*5分钟,将离心后的培养基转移至另一个大离心管中,留一点吹打细胞沉淀成悬液,回收细胞至原培养瓶),若漂浮不严重,亦离心一下。

6)在原培养瓶中留一部分原装培养液,再补加自己新配的培养基至适当容积,例如4ml,让细胞适应一下环境。  当细胞长到70-80%,冻存大部分,小部分传代。

上海慧颖生物专业供应 订购:/!

英文介绍:

Source:

Organ: lung 
Disease: carcinoma; small cell lung cancer

 

Permits/Forms:

In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Pleaseclick here for information regarding the specific requirements for shipment to your location.

    

Receptors:

insulin-like growth factor II (IGF II)

  

Tumorigenic:

Yes

  

Oncogene:

myc +; myb +; fes +; fms +; raf +; ras +

  

DNA Profile (STR):

CSF1PO: 10, 12 
D13S317: 12 
D16S539: 11 
D5S818: 11, 13 
D7S820: 9 
THO1: 8, 9 
TPOX: 10 
vWA: 16, 17 
Amelogenin: XY

  

Cytogenetic Analysis:

modal number = 76 to 78; range = 40 to 87
This is an aneuploid human male cell line. Monosomy of many of the normal chromosomes is noted as well as bisomy in this subtetraploid cell line; however, translocations and deletions involving many of the missing chromosomes are noted, and these chromosomal rearrangements appear to be stable and generally paired. Twelve marker chromosomes were identified including: der(16)t(1;16)(q21;q23), der(22)t(4;22)(q12;q13), der(12)t(11;12)(q23;p12), del(17)(p11), der(19)t(5;19)(?q21;q13) and others.

  

Isoenzymes:

AK-1, 1 
ES-D, 2 
G6PD, B 
GLO-I, 1-2 
Me-2, 1 
PGM1, 2 
PGM3, 1

  

Age:

55 years adult

  

Gender:

male

  

Ethnicity:

Caucasian

  

Comments:

This cell line is aneuploid, will form colonies in soft agar and retains small cell carcinoma morphology and ultrastructure as well as APUD cell characteristics. 
The cells grow in aggregates, thus cell counts are not accurate. 
The cells stain positively for cytokeratins. 
The line can be adapted to grow in shaker flask or spinner flask systems. 
The N-myc gene is amplified, and there is expression of the mRNA and protein. 
C-myc mRNA, but not protein, is expressed at a low level. 
There is expression of c-myb, v-fes, v-fms, c-raf 1, Ha-ras, Ki-ras and N-ras mRNA.

  

Propagation:

ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C

  

Subculturing:

Protocol: Attached cells can be detached by shaking flask Allow aggregates to settle to the bottom of the flask, remove and discard the supernatant medium. Add fresh medium, disperse cells by gentle pipetting and dispense into new flasks. Do not break down aggregates. Subculture every 6 to 8 days. 
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended 
Medium Renewal: 2 times weekly

  

Preservation:

Freeze medium: Complete growth medium, 95%; DMSO, 5% 
Storage temperature: liquid nitrogen vapor temperature

  

Related Products:

Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC30-2001
recommended serum:ATCC 30-2020
derivative:ATCC CRL-11351
purified DNA:ATCC HTB-119D

  

References:

1805: Little CD, et al. Amplification and expression of the c-myc oncogene in human lung cancer cell lines. Nature 306: 194-196, 1983. PubMed: 6646201
22250: Bepler G, et al. Expression of p64c-myc and neuroendocrine properties define three subclasses of small cell lung cancer. Oncogene 4: 45-50, 1989. PubMed: 2536917
22446: Schardt C, et al. Characterization of insulin-like growth factor II receptors in human small cell lung cancer cell lines. Exp. Cell Res. 204: 22-29, 1993. PubMed: 8380141
22465: Broers JL, et al. Spontaneous changes in intermediate filament protein expression patterns in lung cancer cell lines. J. Cell Sci. 91: 91-108, 1988. PubMed: 2473086
22869: Rygaard K, et al. Expression of myc family oncoproteins in small-cell lung-cancer cell lines and xenografts. Int. J. Cancer 54: 144-152, 1993. PubMed: 8386707
23036: Gazdar AF, et al. Establishment of continuous, clonable cultures of small-cell carcinoma of lung which have amine precursor uptake and decarboxylation cell properties. Cancer Res. 40: 3502-3507, 1980. PubMed:6108156
23037: . . Cancer Res. 40: 4556-4563, 1980.
23056: Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257
23057: Gazdar AF, et al. Characterization of variant subclasses of cell lines derived from small cell lung cancer having distinctive biochemical, morphological, and growth properties. Cancer Res. 45: 2924-2930, 1985. PubMed: 2985258
23065: Kiefer PE, et al. Amplification and expression of protooncogenes in human small cell lung cancer cell lines. Cancer Res. 47: 6236-6242, 1987. PubMed: 2824028
23080: Hensel CH, et al. Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer. Cancer Res. 50: 3067-3072, 1990. PubMed: 2159370
24389: . . Lung Cancer 4: 155-161, 1988.
32276: Cairns P, et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 9393760
32488: Geiger T, et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241

 

 

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