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sup-b15细胞,ATCC人Ph+急淋白血病细胞

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  • 产品时间:2017-06-30
  • 简要描述:慧颖生物供应美国ATCCsup-b15细胞人Ph+急淋白血病细胞(ATCC CRL-1929),C3-C5代数,悬浮样,器官来源:骨髓,淋巴样,状态良好,价格优惠,提供Z专业技术指导,提供免费复苏服务,售后齐全,敬请放心购买!
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sup-b15细胞,ATCCPh+急淋白血病细胞 简介:

产品名称:sup-b15细胞

中文名称:人Ph+急淋白血病细胞

来源:美国ATCC

货号:ATCC CRL-1929

类别:细胞系,传代细胞

种属:人

生长状态:悬浮生长

年限:8 years

细胞形态:淋巴样

数量:大量

细胞类型:B淋巴细胞

是否是肿瘤细胞:0

相关疾病:白血病

运输方式:冻存运输

器官来源:骨髓

代数:C3-C5

sup-b15细胞,ATCCPh+急淋白血病细胞 英文描述:

Designations:

SUP-B15

 

Depositors:

 SD Smith

 

Biosafety Level:

1

 

Shipped:

frozen

 

Medium & Serum:

See Propagation

 

Growth Properties:

suspension

 

Organism:

Homo sapiens

 

Morphology:

lymphoblast

 

Source:

Organ: bone marrow 
Disease: acute lymphoblastic leukemia 
Cell Type: B lymphoblast;

 

Cellular Products:

immunoglobulin (cytoplasmic)

 

Permits/Forms:

In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

 

 

 

 

Antigen Expression:

CD1a -; CD2 -; CD3 -; CD4 -; CD5 -; CD8 -; CD10 +; CD13 +; CD38 +; CD71 +; HLA DR +

 

 

DNA Profile (STR):

Amelogenin: X,Y 
CSF1PO: 11,12 
D13S317: 8,14 
D16S539: 11,12 
D5S818: 12,13 
D7S820: 10,11 
THO1: 6,9.3 
TPOX: 8,9 
vWA: 15,17

 

 

Cytogenetic Analysis:

46, XY; the following markers are present: t(9;22)(q34;q11), t(4;14) (p11;q24), der(4)t(1;4) (p11;q33), t(9;22) (q34;q11), der(10)t(3;10) (q25;q26), add(16); Philadelphia chromosome is present.

 

 

Age:

8 years

 

 

Gender:

male

 

 

Ethnicity:

Caucasian

 

 

Comments:

This line was was derived from malignant cells collected from the bone marrow of an 8 year old child with Philadelphia chromosome positive B cell ALL. 
The cells express multiple B lineage markers, but do not express T cell markers. 
The cells are positive for the beta-2-microglobulin, Leu12, My7 (CD13), OKT9 (CD71), OKT10 (CD38) and CALLA (CD10) antigens. [23068 ] 
They are are negative for CB1, Leu 1 (CD5), Leu2 (CD8), Leu3 (CD4), Leu4 (CD3), Leu5 (CD2), Leu6 (CD1a), Leu9, Leu M1 (CD15), My9 (CD33), surface immunoglobulin (sIg -) and Epstein-Barr virus. [23068 ]

 

 

Propagation:

ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 80%; fetal bovine serum, 20%
Temperature: 37.0°C

 

 

Subculturing:

Protocol: Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 5 X 10 exp5 viable cells/ml and maintain between 5 X 10 exp5 and 2 X 10 exp6 cells/ml. 
Medium Renewal: 2 to 3 times per week

 

 

Preservation:

Freeze medium: Complete growth medium 95%; DMSO, 5% 
Storage temperature: liquid nitrogen vapor temperature

 

 

Doubling Time:

18 hrs

 

 

Related Products:

Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020

 

 

References:

22924: Fainstein E, et al. A new fused transcript in Philadelphia chromosome positive acute lymphocytic leukaemia. Nature 330: 386-389, 1987. PubMed: 2825022
22958: Clark SS, et al. Expression of a distinctive BCR-ABL oncogene in Ph1-positive acute lymphocytic leukemia (ALL). Science 239: 775-777, 1988. PubMed: 3422516
23068: Naumovski L, et al. Philadelphia chromosome-positive acute lymphoblastic leukemia cell lines without classical breakpoint cluster region rearrangement. Cancer Res. 48: 2876-2879, 1988. PubMed: 3162827
23302: Rubin CM, et al. Heterogeneity of genomic fusion of BCR and ABL in Philadelphia chromosome-positive acute lymphoblastic leukemia. Proc. Natl. Acad. Sci. USA 85: 2795-2799, 1988. PubMed: 2833755
23305: Kawasaki ES, et al. Diagnosis of chronic myeloid and acute lymphocytic leukemias by detection of leukemia-specific mRNA sequences amplified in vitro. Proc. Natl. Acad. Sci. USA 85: 5698-5702, 1988. PubMed: 3165197

    

 

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