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143B细胞,ATCC人骨肉瘤细胞(CRL-8303)

  • 产品型号:
  • 产品时间:2020-06-17
  • 简要描述:慧颖生物专业供应143B细胞,ATCC人骨肉瘤细胞(CRL-8303)细胞系,成纤维细胞,提供的细胞状态良好,饱满,光泽度好。慧颖生物拥有完善的细胞库管理体系,售后及时到位。另有各类纯进口胎牛血清,培养基,双抗、胰酶,DMSO,生长因子等相关培养产品,种类齐全,价格优惠。!
  • 产品简介

产品名称:143B细胞,人骨肉瘤细胞系

来源:ATCC

货号:CRL-8303

器官来源:骨

生长状态:贴壁生长

规格:株

运输状态:冻存或常温

相关疾病:骨肉瘤

价格:致电咨询

143B细胞,ATCC人骨肉瘤细胞(CRL-8303),详细培养资料及价格,点击

来源:慧颖细胞库

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细胞来源背景介绍:

Designations:

143B

Depositors:

 Wistar Institute

Biosafety Level:

1

Shipped:

frozen

Medium & Serum:

See Propagation

Growth Properties:

adherent

Organism:

Homo sapiens

Morphology:

mixed


Source:

Organ: bone 
Disease: osteosarcoma

Permits/Forms:

In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

 

 

 

 

Applications:

transfection host

 

 

DNA Profile (STR):

Amelogenin: X 
CSF1PO: 12 
D13S317: 12 
D16S539: 10,13 
D5S818: 13 
D7S820: 11,12 
THO1: 6 
TPOX: 11 
vWA: 18

 

 

Age:

13 year old

 

 

Gender:

female

 

 

Ethnicity:

Caucasian

 

 

Comments:

Thymidine kinase negative (TK-). 
This is a human osteosarcoma cell line.

 

 

Propagation:

ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with 0.015 mg/ml 5-bromo-2'-deoxyuridine, 90%; fetal bovine serum, 10%
Temperature: 37.0°C

 

 

Subculturing:

Medium Renewal: 2 to 3 times per week 
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution and allow the cells to sit at room temperature (or at 37C) until they detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

 

 

Preservation:

90% FBS; 10% DMSO

 

 

Related Products:

derivative:ATCC CRL-8304

 

 

References:

32372: Berson JF, et al. A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus tyep 1 strains. J. Virol. 70: 6288-6295, 1996. PubMed: 870925632519: Roller RJ, et al. Structure and function in the herpes simplex virus 1 RNA-binding protein US11: mapping of the domain required for ribosomal and nucleolar association and RNA binding in vitro. J. Virol. 70: 2842-2851, 1996. PubMed: 862775833047: Hofhaus G, et al. Respiration and growth defects in transmitochondrial cell lines carrying the 11778 mutation associated with Leber's hereditary optic neuropathy. J. Biol. Chem. 271: 13155-13161, 1996. PubMed: 866275733152: Hocking AM, et al. Eukaryotic expression of recombinant biglycan. J. Biol. Chem. 271: 19571-19577, 1996. PubMed: 8702651

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