143B细胞，ATCC人骨肉瘤细胞（CRL-8303）

143B

Depositors:

1

Shipped:

frozen

Medium & Serum:

Growth Properties:

adherent

Organism:

Homo sapiens

Morphology:

mixed

Source:

Organ: bone 
Disease: osteosarcoma

Permits/Forms:

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Applications:

transfection host

DNA Profile (STR):

Amelogenin: X 
CSF1PO: 12 
D13S317: 12 
D16S539: 10,13 
D5S818: 13 
D7S820: 11,12 
THO1: 6 
TPOX: 11 
vWA: 18

Age:

13 year old

Gender:

female

Ethnicity:

Caucasian

Comments:

Thymidine kinase negative (TK-). 
This is a human osteosarcoma cell line.

ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with 0.015 mg/ml 5-bromo-2'-deoxyuridine, 90%; fetal bovine serum, 10%
Temperature: 37.0°C

Subculturing:

Medium Renewal: 2 to 3 times per week 
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution and allow the cells to sit at room temperature (or at 37C) until they detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Preservation:

90% FBS; 10% DMSO

Related Products:

derivative:ATCC CRL-8304

References:

32372: Berson JF, et al. A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus tyep 1 strains. J. Virol. 70: 6288-6295, 1996. PubMed: 870925632519: Roller RJ, et al. Structure and function in the herpes simplex virus 1 RNA-binding protein US11: mapping of the domain required for ribosomal and nucleolar association and RNA binding in vitro. J. Virol. 70: 2842-2851, 1996. PubMed: 862775833047: Hofhaus G, et al. Respiration and growth defects in transmitochondrial cell lines carrying the 11778 mutation associated with Leber's hereditary optic neuropathy. J. Biol. Chem. 271: 13155-13161, 1996. PubMed: 866275733152: Hocking AM, et al. Eukaryotic expression of recombinant biglycan. J. Biol. Chem. 271: 19571-19577, 1996. PubMed: 8702651