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产品名称:GP2d细胞,人结肠癌细胞系
同类人结肠癌细胞系有:SW480细胞、SW48细胞、Caco2细胞、SW620细胞、HCT116细胞、 HCT8细胞、HT29细胞、LS174T细胞、LOVO细胞、GP2d细胞
关于 GP2d细胞,人结肠癌细胞系 详细介绍有:
Medium & See Propagation
Organism: Homo sapiens (human)
Source: Organ: colon
Disease: colorectal adenocarcinoma
Morphology: epithelial
Growth adherent
Properties:
Cellular carcinoembryonic antigen (CEA)
Products:
Products
Age: 71 years
Gender: female
Propagation: The base medium for this cell line is formulated RPMI-1640 Medium. To make the complete
growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C
Subculturing: Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 1 to 5 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37癈 to facilitate dispersal.
4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C
Preservation: culture medium 95%; DMSO, 5%
来源:慧颖生物实验室
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